ZFIN Image: Jamalpoor et al., 2021, E

Image

Figure Caption

E Bicalutamide–cysteamine combination treatment corrects the metabolome and proteome profile of cystinotic proximal tubule cells

A

Metabolomic analysis of CRISPR‐generated cystinotic cells (CTNS^−/−) treated with cysteamine (100 µM), bicalutamide (35 µM) and a combination of cysteamine and bicalutamide (100 and 35 µM, respectively) (n = 3).

B

Heat map analysis of the measured metabolites in CTNS^−/− cells upon treatment with cysteamine or cysteamine–bicalutamide combination treatment (n = 3). Metabolites significantly decreased were displayed in green, while metabolites significantly increased were displayed in red.

C, D

Heat map and REACTOME analysis of the altered proteins in CTNS^−/− cells upon treatment with cysteamine, bicalutamide and cysteamine–bicalutamide combination treatment (n = 3). Proteins significantly decreased were displayed in green, while metabolites significantly increased were displayed in red.

E

Proteomic analysis of CTNS^−/− cells treated with cysteamine, bicalutamide, and a combination of cysteamine and bicalutamide, respectively (n = 3). AKGDH: Alpha‐ketoglutarate dehydrogenase, GLUD1: GLUD2; Mitochondrial glutamate dehydrogenase 1/2, IGF2R; Cation‐independent mannose‐6‐phosphate receptor, GSTK1: Glutathione S‐transferase kappa 1, COX6B1: Cytochrome c oxidase subunit 6B1, ACACA: Acetyl‐CoA carboxylase 1‐Biotin carboxylase, GLS: Glutaminase kidney isoform, mitochondrial, CASP3: Caspase‐3, NPC1: Niemann‐Pick C1 protein.

Data information: Data are expressed as mean ± SEM. P‐values < 0.05 were considered to be significant. One‐way ANOVA with Dunnett’s correction (A,B, C, D and E). * significantly different from CTNS^WT cells (P < 0.05). # significantly different from CTNS^−/− cells (P < 0.05). Exact P‐values and statistical tests are listed in Appendix Table S1.

Acknowledgments

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