FIGURE

Fig 5

ID

ZDB-FIG-210714-26

Publication

Pottie et al., 2021 - Loss of zebrafish atp6v1e1b, encoding a subunit of vacuolar ATPase, recapitulates human ARCL type 2C syndrome and identifies multiple pathobiological signatures

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Fig 5

A<italic toggle='yes'>tp6v1e1b</italic> depletion leads to higher amounts of sphingolipids and phospholipids in zebrafish larvae.

(A) Representative images of ultrathin sections of the yolk from 4 dpf WT control and atp6v1e1b-deficient zebrafish. Atp6v1e1b-deficient larvae reveal an accumulation of electron-dense vesicular bodies. Higher magnification of the lipid whorls is shown in the top right corner of the corresponding image. Scale bar = 500 nm (low magnification), scale bar = 200 nm (high magnification). Results are representative of three independent experiments. Mit: mitochondria; endo: endosomal derived multilamellar bodies; lip: lipid whorls. (B-L) HILIC LC-MS/MS lipidomic analysis of atp6v1e1b-deficient zebrafish and WT controls at 3 dpf. Data are expressed as mean ± SD from 3 biological replicates. PC: phosphatidylcholine; PC-O: 1-alkyl,2-acylphosphatidylcholine; PC-P: 1- alkenyl,2-acylphosphatidylcholine; LPC: lysophosphatidylcholine; PG: phosphatidylglycerol; PI: phosphatidylinositol; SM: sphingomyelin; CER: ceramides; DCER: dihydroceramides; HexCer: hexosylceramides; LacCer: lactosylceramides.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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