Figure 1
- ID
- ZDB-FIG-210630-46
- Publication
- Dehmelt et al., 2021 - Spherical arena reveals optokinetic response tuning to stimulus location, size, and frequency across entire visual field of larval zebrafish
- Other Figures
-
- Figure 1
- Figure 1—figure supplement 1.
- Figure 1—figure supplement 2.
- Figure 2
- Figure 2—figure supplement 1.
- Figure 2—figure supplement 2.
- Figure 3
- Figure 3—figure supplement 1.
- Figure 3—figure supplement 2.
- Figure 3—figure supplement 3.
- Figure 3—figure supplement 4.
- Figure 3—figure supplement 5.
- Figure 4
- Figure 4—figure supplement 1
- Figure 4—figure supplement 2.
- Figure 4—figure supplement 3.
- Figure 5
- Figure 5—figure supplement 1.
- Figure 5—figure supplement 2.
- Figure 5—figure supplement 3.
- Figure 6—figure supplement 1.
- Figure 6—figure supplement 1.
- All Figure Page
- Back to All Figure Page
(a) When presented with a horizontal moving stimulus pattern, zebrafish larvae exhibit optokinetic response (OKR) behaviour, where eye movements track stimulus motion to minimise retinal slip. Its slow phase is interrupted by intermittent saccades, and even if only one eye is stimulated (solid arrow), the contralateral eye is indirectly yoked to move along (dashed arrow). (b) Often, experiments on visuomotor behaviour such as OKR sample only a small part of the visual field, whether horizontally or vertically. As different spatial directions may carry different behavioural importance, an ideal stimulation setup should cover all or most of the animal’s visual field. For zebrafish larvae, this visual field can be represented by an almost complete unit sphere. (c) We arranged 232 LED tiles with 64 LEDs each across a spherical arena, such that 14,484 LEDs (green dots) covered nearly the entire visual field. (d) The same individual positions, shown in geographic coordinates. Each circle represents a single LED. Each cohesive group of eight-by-eight circles corresponds to the 64 LEDs contained in a single tile. (e) To identify LED and stimulus locations, we use Up-East-North geographic coordinates: Azimuth α describes the horizontal angle, which is zero in front of the animal and, when seen from above, increases for rightward position. Elevation β refers to the vertical angle, which is zero throughout the plane containing the animal, and positive above. (f) The spherical arena is covered in flat square tiles carrying 64 green LEDs each. (g) Its structural backbone is made of a 3D-printed keel and ribs. Left and right hemispheres were constructed as separate units. (h) Across 85-90% of the visual field, we can then present horizontally moving bar patterns of different location, frequency and size to evoke OKR. |