Behavioral changes on zebrafish 8 dpf old larvae, following 24 h waterborne exposure to 5 µM Deprenyl, 0.5 µM Fluoxetine, 2.5 mM PCPA, and the combination of 2.5 mM PCPA with either Deprenyl 5 µM or Fluoxetine 0.5 µM. (A) Basal locomotor (BLM) activity, represented as the total distance (cm) travelled during 10 min (n = 126–132); (B) acoustic/vibrational escape response (startle), represented as the total distance (cm) travelled following the delivery of a tapping stimulus (n = 127–133); (C) visual-motor response (VMR), representing the response of larvae due to transition of light to dark, represented as the difference of the total distance (cm) travelled by larvae during two minutes after and before the transition of light to dark (n = 121–126); (D) habituation of the acoustic/vibrational escape response evoked by a series of 50 tapping stimulus delivered every second represented as area under the curve (AUC) of larvae responses (n = 124–132). Data are from 3 independent experiments and are reported as scatter plots with the median (red line). Significance was set to p < 0.05 and can be represented as * when p < 0.05; ** when p < 0.01 and *** when p < 0.001, Kruskal–Wallis non-parametric test.
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