|
Ocular expression of Cx35.5 (<italic>gjd2a</italic>) and Cx35.1 (<italic>gjd2b</italic>).Immunostaining showing the topographic distribution of Cx35.5 (gjd2a) and Cx35.1 (gjd2b) throughout the 2.5 mpf zebrafish retina (a)–(t) and Cx35.1 (gjd2b) throughout the 6 mpf zebrafish lens (u). The retinal sections in the left and right column are stained for respectively anti-pan-Cx35 and anti-Cx35.5 (gjd2a) (both in red) and each row indicates the genotype. DAPI (blue) and anti-actin (green) are used for orientation. The staining in the WT retina for anti-pan-Cx35 (a)–(d) and the specific anti-Cx35.5 (e)–(h) reveal generally overlapping patterns of localization in the IPL, OPL, and photoreceptor layer. In the gjd2a (Cx35.5) mutants the anti-pan-Cx35 staining (i)–(l) supports that this antibody recognizes both Cx35.5 (gjd2a) and Cx35.1 (gjd2b) and reveals the localization of Cx35.1 (gjd2b). The absence of staining in the gjd2a (Cx35.5) mutants with the anti-Cx35.5 antibody (m)–(p) support the specificity of the antibody and confirms the Cx35.5 (gjd2a) localization presented in (e)–(h). The presence of staining in the gjd2b (Cx35.1) mutants with the anti-pan-Cx35 antibody (q)–(t) support that this antibody recognizes both Cx35.5 (gjd2a) and Cx35.1 (gjd2b) and supports the localization of Cx35.5 (gjd2a). u Anti-pan-Cx35 immunostaining in isolated 6mpf WT and Cx35.1 (gjd2b) mutant lenses. A modest lenticular appearance at the outer cortical layer can be observed in WT control while absent in gjd2b (Cx35.1) null mutants. The gjd2b (Cx35.1) null mutant lens shows a nuclear ring structure (arrows). Scale bars: 5 µm (a)–(t), 100 µm (u). IPL inner plexiform layer, OPL outer plexiform layer.
|
