FIGURE

Fig. 2

ID

ZDB-FIG-210611-37

Publication

Quint et al., 2021 - Loss of Gap Junction Delta-2 (GJD2) gene orthologs leads to refractive error in zebrafish

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Fig. 2

Opposite refractive error in <italic>gjd2a</italic> (Cx35.5) and <italic>gjd2b</italic> (Cx35.1) mutants.

a Schematic illustration of the eccentric photorefractor setup. b Calibration by −6 diopter (D), −2D, 0D, +4D, and +10D lenses led to a conversion factor of 1.924 (R² = 0.971). c Typical intensity profile of a hyperopic (asterisk) 3mpf gjd2a (Cx35.5) mutant and myopic (arrowhead) lrp2 mutant. The gjd2b (Cx35.1) mutant shows both myopic (arrowhead) and hyperopic (asterisk) contralateral features. d, e RE in the gjd2a (Cx35.5) (d) and gjd2b (Cx35.1) (e) mutants at 1.5 mpf, 2 mpf, 3 mpf, and 9 mpf. d Loss of Cx35.5 (gjd2a) results in a significant (p < 0.001) and progressive hyperopic shift in refractive status. e Loss of Cx35.1 (gjd2b) is linked to a significant (p < 0.001) and progressive myopic shift. f Mutant refractive status normalized against the baseline refraction of WT controls, indicated by the relative RE. Sample size: n = 20 eyes for each genotype and age. Error bars: SEM. Significance: ns = not significant, *p < 0.05, **p < 0.01, ***p < 0.001. Scale bars: 50 µm (c). RE refractive error.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gjd2a^fh437/fh437 gjd2b^fh454/fh454
Observed In:	eye
Stage Range:	Days 45-89 to Adult

Phenotype Detail

Acknowledgments

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