FIGURE 3

NTT results for TMN, clorgyline, and pargyline. Representative heatmaps for (A) TMN (30 mg/L) (B) clorgyline (100 mg/L), and (C) pargyline (10 mg/L) during the NTT for one zebrafish per compound are shown. The heatmaps show the minimum (dark blue) to maximum (dark red) amount of time a fish spent in each pixel. Time spent at the top and the bottom of the tank are quantified for (D and G) TMN (3, 10, and 30 mg/L), (E and H) clorgyline (10, 30, and 100 mg/L), and (F and I) pargyline (10, 30, and 100 mg/L). All compounds show anxiolytic-like effects at at least one of the concentrations and time point. Black dashed line = vehicle control; light blue = lowest concentration; blue = middle concentration; purple = highest concentration. The sample size was 12 except for control group for TMN (n = 11), 3 mg/L TMN (n = 9), 10 mg/L and 30 mg/L TMN (n = 7), 30 mg/L Clorgyline (n = 8), and 100 mg/L Clorgyline (n = 6). Data are expressed as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; Abbreviations: NTT = novel tank test; TMN = 2,3,6-trimethyl-1,4-naphthoquinone.