FIGURE 2

NTT results for harmane, norharmane, and TIQ. Representative heatmaps for (A) harmane (3 mg/L) (B) norharmane (3 mg/L), and (C) TIQ (100 mg/L) during the NTT for one zebrafish per compound are shown. The heatmaps show the minimum (dark blue) to maximum (dark red) amount of time a fish spent in each pixel. Time spent at the top and the bottom of the tank are quantified for (D and G) harmane (1, 3, and 10 mg/L), (E and H) norharmane (0.3, 1, and 3 mg/L), and (F and I) TIQ (10, 30, and 100 mg/L). All compounds show anxiolytic-like effects at at least one of the concentrations and time point. Black dashed line = vehicle control; light blue = lowest concentration; blue = middle concentration; purple = highest concentration. The sample size was 12 except for 10 mg/L harmane, control group for norharmane, 3 mg/L norharmane, and 100 mg/L TIQ, which were n = 11. Data are expressed as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; Abbreviations: NTT = novel tank test; TIQ = 1.2,3,4-tetrahydroisoquinoline.