FIGURE

Fig. 6

ID

ZDB-FIG-210603-10

Publication

Kim et al., 2020 - Investigation of the role of VHL-HIF signaling in DNA repair and apoptosis in zebrafish

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Fig. 6

p53 expression in the vhl?/?; vll?/? double mutants is downregulated in comparison to that in their siblings. (A and B) Embryos from vhl+/?; vll?/? pair mating were collected and irradiated at 2dpf with ?-ray at a dose of 20 gray. q-PCR was performed 24 hours post ?-ray to quantify the full length of p53 and short form of ?113p53. This revealed that there was a significant decrease in both forms of p53 expression in the vhl?/?; vll?/? embryos in comparison to their siblings. ****p < 0.0001, two way ANOVA test. The statistical analysis was performed with ddCt values. (C) We also treated embryos from vhl+/?; vll?/? incross with 10 nM CPT and both isoforms of p53 induction was examined in the vhl?/?; vll?/? embryos and in their siblings by Western blot. After treatment, there was a clear reduction in both isoforms of p53 expression in the vhl?/?; vll?/? embryos in comparison to their siblings.

Expression Data

Gene:	tp53
Fish:	vhl^{hu2117/hu2117}; vhll^i216/i216; sh144Tg
Anatomical Term:	whole organism
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vhl^{hu2117/hu2117}; vhll^i216/i216; sh144Tg
Observed In:	whole organism
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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