FIGURE

Fig. 5

ID

ZDB-FIG-210518-83

Publication

Sheets et al., 2021 - How Zebrafish Can Drive the Future of Genetic-based Hearing and Balance Research

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Fig. 5

Outline of how to create germline zebrafish CRISPR-Cas9 mutants and CRISPants. To create a germline zebrafish CRISPR-Cas9 mutant (follow path of solid lines), guide RNAs (gRNAs) targeting a gene of interest along with Cas9 mRNA or protein are injected into newly fertilized zebrafish embryos. These G₀ injected embryos are grown to adulthood (2–3 months). G₀ adult founders are crossed to wildtype adults, and the G₁ progeny screened for indels. G₁ progeny with indels are grown to adulthood. G₁ adults containing indels are then incrossed and screened for phenotypes. To perform analyses on injected G₀ CRISPant larvae, optimized gRNAs are injected into newly fertilized zebrafish embryos. Injected CRISPant G₀ larvae are then screened for phenotypes days after the injection. CRISPant phenotypes can be verified by generating a stable, germline mutant

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Assoc. Res. Otolaryngol.