FIGURE

Figure 4.

ID

ZDB-FIG-210510-9

Publication

Stevenson et al., 2021 - Giantin is required for intracellular N-terminal processing of type I procollagen

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Figure 4.

Procollagen processing is defective in giantin KO RPE1 cells. (A) Representative images of WT and giantin KO cells expressing GFP-COL1A1 or GFP alone as indicated and immunolabeled for pro-α1(I) (COL1A1, magenta). Cells are not expressing a RUSH hook. Nuclei are stained with DAPI (blue). Images are maximum-intensity projections of widefield z-stacks. Scale bars = 10 µm. (B–F) Immunoblots of secretion assays showing the medium (M) and lysate (L) fractions of WT and giantin KO cell cultures immunoblotted for GFP and tubulin (B), SBP and tubulin (C), pro-α1(I) C-propeptide (LF41 antibody) and tubulin (D), pro-α1(I) N-propeptide (LF39 antibody; E), and pro-α1(I) and GAPDH (F). Black arrows indicate full-length procollagen, and red arrows highlight N-propeptide bands. (G) Maximum-intensity projection widefield z-stacks of WT and giantin KO cells stably expressing GFP-COL1A1 immunolabeled for the pro-α1(I) N-propeptide (LF39 antibody). Scale bars = 10 µm. Ab, antibody.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Biol.