FIGURE

Figure 4

ID

ZDB-FIG-210508-42

Publication

Magnussen et al., 2021 - Identification of a Catalytic Active but Non-Aggregating MDM2 RING Domain Variant

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Figure 4

Systematic analysis of MDM2 RING domain aggregation across species (a, c, e, g, i, k, m, o) Superdex 75 elution profiles of MDM2^h, MDM2^f, opossum MDM2 421-C, galago MDM2 425-C, MDM2^h A434S, MDM2^h I435V, MDM2^h G443T and MDM2^h P491S, respectively (shown as black solid line). The elution profile of molecular weight markers (a: bovine serum albumin, 66 kDa; b: carbonic anhydrase, 29 kDa; c: cytochrome C, 12.4 kDa; d: aprotinin, 6,5 kDa) is shown as red dashed line. GST-MDM2 variants (expressed from 2L LB) were treated with TEV then loaded on a Superdex 75 Increase 10/300 column. (b, d, f, h, j, l, n, p) SDS-PAGE showing the GST-MDM2 variants before and after TEV treatment to release the GST-tag (labeled ‘–’ and ‘+’, respectively) and single fractions of the corresponding SEC experiments from panels a, c, e, g, i, k, m, o, respectively. The position of the fractions within the elution profile is indicated by numbers (1–5).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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