Fig. 5

FdmR deficiency results in synthesis of shortened lipids and increased cell-wall permeability. (A) ¹³C fractional labeling of fatty acids in M. marinum wild type and ∆fdmR mutant grown on [U-¹³C]acetate. (B) Ratio of fatty acid contents in the ∆fdmR mutant versus wild type during growth on oleate, acetate, or propionate. Gray color indicates that fatty acid is below detection limits. Data are means of three independent experiments, normalized to the values in the wild type. (C) Relative abundance of major lipids in M. marinum ∆fdmR mutant compared to the wild type. The strains were grown in 7H9 with glycerol and OADC. Abbreviations of phospholipids: PE, phosphatidylethanolamine; PI, phosphatidylinositol; PG, phosphatidylglycerol; PA, phosphatidic acid; PS, phosphatidylserine; PIM, phosphatidylinositol mannoside; CL, cardiolipin. (D) Fractions of relatively short and long species within α-TMM and oxygenated TMM in the wild type and ∆fdmR mutant. (E) Effect of FdmR deficiency on M. marinum cell-wall permeability determined by ethidium bromide accumulation assay. Data shown in A, C, D, and E are mean ± SD (n = 3 independent experiments). **P < 0.01, ***P < 0.001, not significant (ns), by unpaired two-tailed t test.