Fig. 4

FdmR deficiency results in overactive catabolism of fatty acids. (A) Kinetics of labeling of oleoyl-CoA and acetyl-CoA after switching M. marinum cells to [U-¹³C]oleate medium. Data shown are mean ± SD (n = 3 independent experiments). (B) In vivo fluxes in fatty acid catabolism in M. marinum wild type and ∆fdmR mutant. ISOCIT, isocitrate; GLO, glyoxylate; SUC, succinate; MAL, malate. (C) In vivo fluxes of acetate assimilation and glyoxylate shunt in wild type and ∆fdmR mutant. (D) In vivo fluxes through methylmalonyl-CoA (MM-CoA) pathway and methylcitrate cycle in wild type and ∆fdmR mutant. The strains were grown on medium containing[U-¹³C]oleate (B), [U-¹³C]acetate (C), or [U-¹³C]propionate (D). Data shown in B, C, and D are median ± 95% confidence interval (n = 3 independent experiments) and are expressed in nanomoles per milligram cell dry weight per minute. (E) Heat map showing the ratio of metabolite concentrations in the ∆fdmR mutant versus wild type during growth on oleate, acetate, or propionate. Data are means of three independent experiments and normalized to the values in the wild type.