FIGURE

Figure 2

ID

ZDB-FIG-210503-91

Publication

Ko et al., 2021 - Differential Regulation of Ca²⁺-Activated Cl^- Channel TMEM16A Splice Variants by Membrane PI(4,5)P₂

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Figure 2

Analysis of PI(4,5)P₂ regulation regarding the two steps of TMEM16A activation. (A) Representative Cl? current traces activated by a voltage step to +50 mV before (control) and after Dr-VSP activation in cells expressing TMEM16A(ac) in the presence of a 115 nM intracellular Ca²⁺ concentration. Black trace: control (before Dr-VSP activation). Red trace: after Dr-VSP activation. The dotted line indicates zero current. The horizontal dashed lines (blue) indicate the separation point for the instantaneous and slow currents. I_Inst?instantaneous Cl^? current; I_Slow?slow Cl^? current. (B) Inhibition (%) of the instantaneous (top) and slow (bottom) currents in response to PI(4,5)P₂ depletion by Dr-VSP activation in cells expressing TMEM16A(a) or TMEM16A(ac); n = 5?7. Dots indicate the individual data points for each cell. Bars indicate means � SEM. ** p < 0.01, *** p < 0.001, one-way ANOVA followed by Sidak?s post hoc test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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