FIGURE

Figure 2

ID

ZDB-FIG-210503-187

Publication

Pfefferli et al., 2021 - Persistent Ventricle Partitioning in the Adult Zebrafish Heart

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Figure 2

FHF lineage labeling persists during developmental stages. (A) Crossing scheme of used transgenes and timeline of experimental setup with 5 days post-fertilization (dpf) embryos and 22 dpf larvae longitudinally sectioned for heart analysis. (B?D) Confocal images of individual coronal sections, stained for pan-muscle Tropomyosin (blue, sm indicating skeletal muscles, v indicating ventricle, a indicating atrium). Embryos at 5 dpf (B), treated with DMSO (control, n = 4) at the 75% epiboly, show only myocardial AmCyan expression from myl7:Switch, while 4-OHT treatment (C) results in ZsYellow-expressing clones from drl:creERT2 activity, as also observed at 22 dpf (D). (E) Quantification of the ZsYellow?Tropomyosin double-positive cardiomyocytes of analyzed ventricles, two-tailed Student?s t-test, * p = 0.0149, 5 dpf n = 6; 22 dpf n = 6. Scale bar 50 �m.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J Cardiovasc Dev Dis