FIGURE

Figure 2

ID

ZDB-FIG-210428-7

Publication

Nogaret et al., 2021 - A Novel Infection Protocol in Zebrafish Embryo to Assess Pseudomonas aeruginosa Virulence and Validate Efficacy of a Quorum Sensing Inhibitor In Vivo

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Figure 2

Validation of bath infection model using an attenuated strain of P. aeruginosa. (A) Kaplan?Meier representation of survival after microinjection of approximately 3500 CFUs of wild-type or ?oprF GFP-expressing P. aeruginosa into the caudal vein (CV) of embryos. Pools of three biologically independent replicates are shown (in each experiment 20 embryos were used per strain). (B) Kaplan?Meier representation of survival after bath infection of injured embryos with wild-type or ?oprF GFP-expressing P. aeruginosa at approximately 10⁸ CFU/mL. Pools of three biologically independent replicates are shown for each survival curve (in each experiment 20 embryos were used per strain).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Condition:	bacterial treatment by exposure to environment: Pseudomonas aeruginosa, transection: caudal fin
Observed In:	whole organism
Stage Range:	Pec-fin to Day 5

Phenotype Detail

Acknowledgments

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