FIGURE

Fig. 1

ID

ZDB-FIG-210419-1

Publication

Don et al., 2021 - In vivo Validation of Bimolecular Fluorescence Complementation (BiFC) to Investigate Aggregate Formation in Amyotrophic Lateral Sclerosis (ALS)

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Fig. 1

TDP-43 aggregation in zebrafish is specific. BiFC assay to determine TDP-43 aggregation. a–c Graphical illustration of the workflow. Male and female zebrafish are set up to collect the fertilized eggs (a). Eggs at the 1–2-cell stage are microinjected (b) with a combination of BiFC mRNA as illustrated below. Embryos are raised and BiFC complementation is visualized using a microscope (c). d–f Top schematics illustrate the different injection combinations. d TDP-43-aggregation and respective fluorescent signal in zebrafish somites at 24 hpf. e TDP-43-independent signal (noise). f Control (background) fluorescence at 24 hpf. Scale bar represents 20 μm. g Comparison of BiFC intensity after standard and competitive TDP-43 BiFC injections. The bar diagram shows the quantitative comparison of the normalized BiFC intensity in this fluorescence complementation assay. Dot points represent individual fish and data were pooled from 4 independent experiments. ****P < 0.0001

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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