FIGURE

Fig. 2

ID

ZDB-FIG-210413-49

Publication

Luo et al., 2020 - Stepwise crosstalk between aberrant Nf1, Tp53 and Rb signalling pathways induces gliomagenesis in zebrafish

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Fig. 2

Disruption of tissue-specific genes in larvae and adult zebrafish. (A) Representative images of various single-deletion mutations, and gfapWT control in 3-month-old adult fish. The fluorescent radial glia phenotype was observed based on mCherry-labelled Gfap expression, which failed to co-localize with Nf1, Rb1 or Tp53 expression in brain tissues of transgenic zebrafish (arrowheads; right), whereas partial co-localizations of mCherry-labelled Gfap with Nf1, Rb1 or Tp53 expression were observed in gfapWT fish (arrows; left). Scale bars = 50 μm. (B) Merged bright and fluorescent images showing mCherry-labelled Gfap expression in the cerebellum and medulla in 3-month-old gfapWT fish. Scale bars = 500 μm. (C) Coronal sections of the images shown in i–iii. Robust mCherry-labelled Gfap expression was observed along the midline and dorsal lining of the fourth ventricle. Scale bars = 50 μm.

Expression Data

Genes:	nf1a nf1b rb1 tp53
Fish:	AB nf1a^{zf3453a/zf3453a}; zf3453Tg (AB)
Anatomical Term:	brain radial glial cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nf1a^{zf3453a/zf3453a}; zf3453Tg (AB)
Observed In:	brain radial glial cell
Stage:	Adult

Phenotype Detail

Acknowledgments

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