FIGURE

F

ID

ZDB-FIG-210409-113

Publication

Acin-Perez et al., 2020 - A novel approach to measure mitochondrial respiration in frozen biological samples

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<styled-content toggle='no' style='fixed-case'>RIFS</styled-content> measurement of Complex I, <styled-content toggle='no' style='fixed-case'>II</styled-content>, and <styled-content toggle='no' style='fixed-case'>IV</styled-content> activity in fresh and frozen liver mitochondria

Representative pyruvate + malate seahorse profile using RIFS the respirometry protocol in mouse liver mitochondria isolated from fresh or frozen tissue. Pyruvate + malate (Pyr + Mal), antimycin A + rotenone (AA + ROT), TMPD + ascorbate (TMPD/Asc), and azide were injected sequentially

Pyruvate + malate‐ and TMPD/ascorbate (Complex IV, CIV)‐dependent respiration in fresh and frozen liver mitochondria.

Representative succinate + rotenone seahorse profile using the RIFS respirometry protocol in mouse liver mitochondria isolated from fresh or frozen tissue.

Succinate + rotenone (Succ)‐ and CIV‐dependent respiration in fresh and frozen liver mitochondria.

Representative NADH seahorse profile using RIFS respirometry protocol in liver mitochondria isolated from fresh or frozen tissue.

NADH‐ and CIV‐dependent respiration in fresh and frozen liver mitochondria.

Data information: Panels (A, C, and E) are representative seahorse traces including four technical replicates. Biological replicates: (B and F), n = 3–4; (D), n = 4–6. Every biological replicate represents the average of four technical replicates. Data are the mean ± SEM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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