FIGURE

F

ID

ZDB-FIG-210409-116

Publication

Acin-Perez et al., 2020 - A novel approach to measure mitochondrial respiration in frozen biological samples

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<styled-content toggle='no' style='fixed-case'>RIFS</styled-content> measurement of Complex I, <styled-content toggle='no' style='fixed-case'>II</styled-content>, and <styled-content toggle='no' style='fixed-case' xmlns:xlink='http://www.w3.org/1999/xlink'>IV</styled-content> activity in fresh and frozen liver homogenates

Representative pyruvate + malate seahorse profile using RIFS respirometry protocol in liver homogenates obtained from fresh or frozen tissue (top panel) and quantification (bottom panel).

Representative succinate + rotenone seahorse profile using RIFS respirometry protocol in liver homogenates obtained from fresh or frozen tissue (top panel) and quantification (bottom panel).

Representative NADH seahorse profile using RIFS respirometry protocol in liver homogenates obtained from fresh or frozen tissue (top panel) and quantification (bottom panel).

NADH‐dependent respiration in frozen liver mitochondria in the presence of phenformin and metformin and the indicated concentration.

Complex I‐, II‐, and IV‐dependent respiration in liver homogenates from WT and Ndufs4 KO mice showed impaired Complex I with normal Complex II and IV respiration in mouse Ndufs4 KO.

Representative Western blot followed by quantification of Complex I (NDUFA9 and NDUFB8) levels in Ndufs4 KO samples. β‐actin was used as loading control.

Data information: Biological replicates: (A and C–F), n = 3–4; (B), n = 4–6. Every biological replicate represents the average of four technical replicates. Data are the mean ± SEM. For calculating statistics, the number of replicates used is the biological replicates indicated above. We used the following tests: two‐way ANOVA with Tukey's multiple comparison test in panels (D and F); unpaired t‐test in panel (E). *P < 0.05; **P < 0.01; ****P < 0.0001.Source data are available online for this figure.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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