ZFIN Image: Acin-Perez et al., 2020, F

Image

Figure Caption

F <styled-content toggle='no' style='fixed-case'>RIFS</styled-content> measurement of Complex I, <styled-content toggle='no' style='fixed-case'>II</styled-content>, and <styled-content toggle='no' style='fixed-case'>IV</styled-content> activity in fresh and frozen liver mitochondria

A

Representative pyruvate + malate seahorse profile using RIFS the respirometry protocol in mouse liver mitochondria isolated from fresh or frozen tissue. Pyruvate + malate (Pyr + Mal), antimycin A + rotenone (AA + ROT), TMPD + ascorbate (TMPD/Asc), and azide were injected sequentially

B

Pyruvate + malate‐ and TMPD/ascorbate (Complex IV, CIV)‐dependent respiration in fresh and frozen liver mitochondria.

C

Representative succinate + rotenone seahorse profile using the RIFS respirometry protocol in mouse liver mitochondria isolated from fresh or frozen tissue.

D

Succinate + rotenone (Succ)‐ and CIV‐dependent respiration in fresh and frozen liver mitochondria.

E

Representative NADH seahorse profile using RIFS respirometry protocol in liver mitochondria isolated from fresh or frozen tissue.

F

NADH‐ and CIV‐dependent respiration in fresh and frozen liver mitochondria.

Data information: Panels (A, C, and E) are representative seahorse traces including four technical replicates. Biological replicates: (B and F), n = 3–4; (D), n = 4–6. Every biological replicate represents the average of four technical replicates. Data are the mean ± SEM.

Acknowledgments

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