Fig. 4

a, b Expression of ifnφ1 (a) and ifnφ3 (b) mRNA after transfected with bmp8a (2 μg) in ZFL cells for 24 h, followed by treatment with SB203580, SP600125, U0126, DMH1, and TP0427736 HCl for another 24 h. c Similar as (a, b) but in EPC cells. The expression of zebrafish actb1 or EPC actin was used as an internal control for the qRT-PCR. d–f EPC cells were cotransfected with IFNφ1pro-luc (200 ng, d), IFNφ3pro-luc (200 ng, e) or EPC IFNpro-luc (200 ng, f), pRL-TK (20 ng) together with bmp8a (200 ng) or empty vector (200 ng), respectively. At 24 h of post-transfection, cells were treated with or without SB203580 for another 24 h and then harvested for detection of luciferase activity. Renilla luciferase was used as the internal control. Data were from three independent experiments and were analyzed by Student’s t-test (two-tailed) for comparison of two groups or one-way ANOVA followed by Games–Howell post hoc tests for comparison of multiple groups. All data were presented as mean ± SD (*p < 0.05, **p < 0.01, and ***p < 0.001).