Figure 7

Neuronal association between Tac1-immunoreactive processes and GnRH3 cell soma in the male and female zebrafish. Photomicrographs for immunofluorescence of GnRH3 (1^st column, A−D), and Tac1 (2^nd column, A’−D’) were merged (3^rd column, A”−D”) to elucidate the possible neuronal association (arrows) between Tac1 and GnRH3 neurons. A representative GnRH3 cell soma accompanied by Tac1 processes was further examined by two-dimensional confocal scanning in XY, XZ, and YZ plane (4^th column, A’”−D’”). Bar graphs in the bottom panel (E) show the percentage of GnRH3 cells accompanied by Tac1 processes in males (blue columns) and females (red columns) in the ventral nucleus of ventral telencephalic area (Vv) and olfactory-terminal nerve (OB-TN) region. Numbers in each columns indicate the number of GnRH3 cells accompanied by Tac1 processes and total GnRH3 numbers counted. In the Vv, more Tac1-immunoreactive processes (magenta) are in proximities of GnRH3 cells (green) in males (A−A’”) as compared to females (C−C’”), but there was no significant difference in the percentage of GnRH3 cells between males and females (E). In the olfactory bulb (OB), OB-GnRH3 neurons are accompanied by Tac1 processes in both males (B−B’”) and females (D−D’”), and there was no significant sex difference in the percentage of GnRH3 cells (E). ns, non-significant. Scale bars: 10 µm.