FIGURE

Figure 7

ID

ZDB-FIG-210325-38

Publication

Guo et al., 2021 - Trio cooperates with Myh9 to regulate neural crest-derived craniofacial development

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Figure 7

myh9 and ca-Rac1/ca-Cdc42 partly restore the defects in trio morphants. (A) Bright field image of zebrafish embryos at 96 hpf. Bar = 100 ?m. Red dotted line: mandible. ?+?, coinjection. MO, morpholino. hpf, hours post-fertilization. c: con MO, t: trio MO, m: myh9 MO, t+m: trio MO+myh9 mRNA, t+R: trio MO+Rac1 mRNA, t+C: trio MO+Cdc42 mRNA, m+R: myh9 MO+Rac1 mRNA, m+C: myh9 MO+Cdc42 mRNA. (B, C) Images and quantification of Alcian blue-stained zebrafish embryos at 120 hpf (n = 10). Bar = 500 ?m. (D) Eye iridophore amount and distribution of the experimental groups (n = 10). Bar = 100 ?m. (E) Time-lapse images of live Tg(sox10:egfp) zebrafish at 14 and 16 SS in the experimental groups mentioned above. Bar = 100 ?m. SS, somite stage. For (B) and (D), data are represented as mean � S.E.M. (two-tailed t test *p < 0.05, **p < 0.01).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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