Figure 7

myh9 and ca-Rac1/ca-Cdc42 partly restore the defects in trio morphants. (A) Bright field image of zebrafish embryos at 96 hpf. Bar = 100 μm. Red dotted line: mandible. “+”, coinjection. MO, morpholino. hpf, hours post-fertilization. c: con MO, t: trio MO, m: myh9 MO, t+m: trio MO+myh9 mRNA, t+R: trio MO+Rac1 mRNA, t+C: trio MO+Cdc42 mRNA, m+R: myh9 MO+Rac1 mRNA, m+C: myh9 MO+Cdc42 mRNA. (B, C) Images and quantification of Alcian blue-stained zebrafish embryos at 120 hpf (n = 10). Bar = 500 μm. (D) Eye iridophore amount and distribution of the experimental groups (n = 10). Bar = 100 μm. (E) Time-lapse images of live Tg(sox10:egfp) zebrafish at 14 and 16 SS in the experimental groups mentioned above. Bar = 100 μm. SS, somite stage. For (B) and (D), data are represented as mean ± S.E.M. (two-tailed t test *p < 0.05, **p < 0.01).