FIGURE

Figure 5.

ID

ZDB-FIG-210319-49

Publication

Jung et al., 2021 - Trim46 contributes to the midbrain development via Sonic Hedgehog signaling pathway in zebrafish embryos

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Figure 5.

Inhibition of SHH signaling by cyclopamine decreased the level of trim46a transcripts in dose-dependent manners. (A–D) WISH analysis using foxa2 specific probe detected foxa2 transcripts in the midbrain at 24 hpf. Level of foxa2 transcripts decreased in the embryos treated with cyclopamine versus the control. (E–H) WISH analysis of cyclopamine-treated embryos using trim46a as a probe. Cyclopamine caused reduction in the level of trim46a transcripts in comparison to those of EtOH control in dose-dependent manners. (I) Quantification of foxa2 transcripts in the ZLI, and trim46a transcripts in the forebrain, midbrain and cerebellum at 24 hpf using ImageJ software. CCP, cyclopamine. (A–H) Scale bars: 50 μm.

Expression Data

Genes:	foxa2 trim46a
Fish:	WT
Condition:	chemical treatment by environment: Cyclopamine
Anatomical Terms:	hypothalamus midbrain midbrain hindbrain boundary zona limitans intrathalamica
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	chemical treatment by environment: Cyclopamine
Observed In:	hypothalamus midbrain midbrain hindbrain boundary zona limitans intrathalamica
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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