FIGURE

Figure 4.

ID

ZDB-FIG-210319-47

Publication

Jung et al., 2021 - Trim46 contributes to the midbrain development via Sonic Hedgehog signaling pathway in zebrafish embryos

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Figure 4.

Inhibition of SHH signaling with cyclopamine resulted in developmental defects in the midbrain of zebrafish embryos at 24?hpf. (A?D) Zebrafish embryos at 24?hpf which were treated 0.2% and 0.6% EtOH from 4?hpf. 0.6% EtOH-exposed embryos showed similar phenotypes of midbrain and MHB in comparison to those of 0.2% EtOH-exposed embryos. (E and F) Cyclopamine-treated embryos (20??M) showed reduction in width of the midbrain and dorsally shrunken midbrain in comparison to those of EtOH controls. (G and H) 60??M cyclopamine-treated embryos exhibited severe defects in the midbrain. (I?L) Spatiotemporal expression patterns of otx2b in the midbrain of cyclopamine-treated embryos at 24?hpf. CCP, cyclopamine. (A?L) Scale bars: 50??m.

Expression Data

Gene:	otx2b
Fish:	WT
Condition:	chemical treatment by environment: Cyclopamine
Anatomical Term:	midbrain
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	chemical treatment by environment: Cyclopamine
Observed In:	midbrain midbrain dorsal region
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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