FIGURE

Figure 4.

ID

ZDB-FIG-210319-47

Publication

Jung et al., 2021 - Trim46 contributes to the midbrain development via Sonic Hedgehog signaling pathway in zebrafish embryos

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Figure 4.

Inhibition of SHH signaling with cyclopamine resulted in developmental defects in the midbrain of zebrafish embryos at 24 hpf. (A–D) Zebrafish embryos at 24 hpf which were treated 0.2% and 0.6% EtOH from 4 hpf. 0.6% EtOH-exposed embryos showed similar phenotypes of midbrain and MHB in comparison to those of 0.2% EtOH-exposed embryos. (E and F) Cyclopamine-treated embryos (20 μM) showed reduction in width of the midbrain and dorsally shrunken midbrain in comparison to those of EtOH controls. (G and H) 60 μM cyclopamine-treated embryos exhibited severe defects in the midbrain. (I–L) Spatiotemporal expression patterns of otx2b in the midbrain of cyclopamine-treated embryos at 24 hpf. CCP, cyclopamine. (A–L) Scale bars: 50 μm.

Expression Data

Gene:	otx2b
Fish:	WT
Condition:	chemical treatment by environment: Cyclopamine
Anatomical Term:	midbrain
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	chemical treatment by environment: Cyclopamine
Observed In:	midbrain midbrain dorsal region
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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