Fig. 1
- ID
- ZDB-FIG-210316-7
- Publication
- Bertozzi et al., 2020 - Is zebrafish heart regeneration "complete"? Lineage-restricted cardiomyocytes proliferate to pre-injury numbers but some fail to differentiate in fibrotic hearts
- Other Figures
- All Figure Page
- Back to All Figure Page
Fig. 1. Cardiomyocyte cell cycle activity drops to low levels prior to complete morphological regeneration. (A) The fraction of PCNA+ EGFP+ cardiomyocytes in myl7:EGFPtwu34 fish within 150 μm of the wound border peaks at 7 dpi and drops to levels barely above baseline by 30 dpi. uninj, uninjured. Error bars represent CI 95%. n = 5 hearts for each group. One-way ANOVA + Dunnett’s multiple comparisons test. Numbers indicate p-value. (B) Fraction of EdU+ EGFP+ cardiomyocytes in myl7:EGFPtwu34 fish. For each time point, a single intraperitoneal injection of EdU was performed 24 h prior to heart extraction. n (hearts) = 5 (uninjured), 5 (3 dpi), 5 (7 dpi), 7 (14 dpi), 8 (30 dpi), 7 (60 dpi). One-way ANOVA + Dunnett’s multiple comparisons test. (C) Many hearts contain wound tissue detected by Acid Fuchsin Orange G (AFOG) staining at 30 dpi. Red: fibrin, blue: collagen, brown: myocardium. Scale bars, 100 μm. (D) Percentage of hearts without residual wounds (= 0% of ventricle area), small wounds (between 0% and 3%) or bigger wounds (>3%) at 14, 30 and 90 dpi. n = 31 (14 dpi), 29 (30 dpi), 58 (90 dpi). Chi-square test. |
Reprinted from Developmental Biology, 471, Bertozzi, A., Wu, C.C., Nguyen, P.D., Vasudevarao, M.D., Mulaw, M.A., Koopman, C.D., de Boer, T.P., Bakkers, J., Weidinger, G., Is zebrafish heart regeneration "complete"? Lineage-restricted cardiomyocytes proliferate to pre-injury numbers but some fail to differentiate in fibrotic hearts, 106-118, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.