ZFIN ID: ZDB-FIG-210316-44
Gray et al., 2020 - Postembryonic screen for mutations affecting spine development in zebrafish. Developmental Biology   471:18-33 Full text @ Dev. Biol.
Observed In:
Stage: Days 30-44

Fig. 6 Generation of a novel kif6 p.P293T mutant zebrafish. (A) Clustal W alignment of Human and Danio rerio Kif6 protein to highlight the P293T mutation (marked by asterisk). (B) Schematic of changes for donor editing of the kif6dp20 allele. (C) Sanger sequence of a stable heterozygous kif6dp20 mutant zebrafish. (D) Representative EcoR1 and allele specific genotyping of the kif6dp20 allele. (E, F) Representative phenotypes of a homozygous kif6dp20 mutant zebrafish at 30 dpf.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
kif6ut20/ut20 standard conditions Days 30-44 axis increased curvature, abnormal
Days 30-44 axis normal length, normal
Days 30-44 vertebral column increased curvature, abnormal
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Reprinted from Developmental Biology, 471, Gray, R.S., Gonzalez, R., Ackerman, S.D., Minowa, R., Griest, J.F., Bayrak, M.N., Troutwine, B., Canter, S., Monk, K.R., Sepich, D.S., Solnica-Krezel, L., Postembryonic screen for mutations affecting spine development in zebrafish, 18-33, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.