Figure 4

TAp73β activates expression and nuclear localization of YAP, but does not affect Bax expression. (A) Hep3BTAp73β cells were treated with doxycycline (1 µg/mL) for 72 h and cell lysates were obtained, then western blot was performed for p73, YAP and Bax expression. β-actin was used as a loading control (n = 2). * Non-specific signals. (B) Upon TAp73β induction, both TAp73 and YAP show nuclear accumulation (*). Immunofluorescence assay was performed to determine the expression and localization of p73 (Red), YAP (Green), respectively in Hep3BTAp73β cells treated with Doxycycline (1 µg/mL) for 72 h. DAPI (blue) was used for nuclear counterstain. Images were taken at x63 magnification in a confocal microscope. Scale bar = 20 μm. * Please note that the antibody used for TAp73β immunofluorescence recognizes all p73 isoforms [27]. Similarly, D24E4 antibody used for YAP immunofluorescence also detects TAZ protein.