Figure 6

TAp73β represses AFP+ hepatocyte-like 3D cell structures formation while stimulating CK19+ cholangiocyte-like 3D cell structures formation. (A) A scheme depicting Hep3BTAp73β cells grown in the absence of doxycycline (DOX−) in Matrigel for 4 days, followed by additional growth in the absence (Dox−) or presence (DOX+) of doxycycline (1 µg/mL) for 5 days. (B) Brightfield images of differentially treated 3D cell structures at days 3 and 5, and H&E staining at day 5. (C) Immunohistochemistry staining for CK19 and AFP which was performed using formaldehyde-fixed paraffin-embedded 3D cell structures that have been grown 5 days in the presence (DOX+) or absence (DOX−) of doxycycline. Images were taken at ×20 magnification in a brightfield microscope. Scale bar = 100 μm.