FIGURE

Fig 6

ID

ZDB-FIG-210304-11

Publication

Lu et al., 2021 - A novel role of Zebrafish TMEM33 in negative regulation of interferon production by two distinct mechanisms

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Fig 6

(A) Schematic representation of full-length TMEM33 and its mutants. (B-D) The first and the second TM regions of TMEM33 are necessary for its association with MAVS, TBK1, and MITA. HEK 293T cells seeded in 10 cm2 dishes were co-transfected with the indicated plasmids (5 ?g each). After 24 h, cell lysates were immunoprecipitated (IP) with anti-Flag affinity gel (B and C) or anti-Myc affinity gel (D). Then the immunoprecipitates and WCLs were analyzed by IB with the indicated Abs. (E-G) Diagrammatic representations of full-length MAVS/TBK1/MITA and deletion mutants in this study. (H) The N-terminal CARD domain and C-terminal TM domain of MAVS are responsible to its interaction with TMEM33. The experiments were performed similarly as described above for panel B. (I) The N-terminal kinase domain of TBK1 is essential to its interaction with TMEM33. The experiments were performed similarly as described above for panel B. (J) TMEM33 associates with MITA via its N terminus. The experiments were performed similarly as described above for panel B. All experiments were repeated for at least three times with similar results.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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