FIGURE

Figure 4

ID

ZDB-FIG-210224-15

Publication

DeMarco et al., 2021 - Pyramidal Neurons of the Zebrafish Tectum Receive Highly Convergent Input From Torus Longitudinalis

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Figure 4

PSD95-positive post-synaptic specializations in PyrN dendrites. (A) Maximum projection of PSD95-EGFP and DsRed expression in an isolated id2b:gal4 positive PyrN. Note discrete enrichments of PSD95-EGFP relative to cytosolic DsRed label. (A?) +50� Y-axis rotated view of image volume in (A). SM, SFGS, and SGC dendritic portions are indicated by boxed regions. Note presence of PSD95-EGFP puncta in SM, SFGS, and SGC layer dendrites. (B) ?40� Y-axis rotated views of isolated subvolumes from boxed regions in (A?) showing PSD95-EGFP and DsRed expression in the SM, SFGS, and SGC dendrites. (C) Threshold images of PSD95-EGFP channel in rotated subvolumes in (B) as an example of threshold values used for automated particle analysis (puncta counting). (D?F) Quantification of PSD95-EGFP puncta number, neurite length, and puncta density for SM, SFGS, and SGC dendrite subregions in 7 fully reconstructed PyrNs obtained from 7 larvae. Scale bar: 20 ?m in (A) and 15 ?m in (B,C). *p < 0.05; **p < 0.001; ANOVA with Tukey's post-hoc test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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