FIGURE

Figure 4

ID

ZDB-FIG-210224-15

Publication

DeMarco et al., 2021 - Pyramidal Neurons of the Zebrafish Tectum Receive Highly Convergent Input From Torus Longitudinalis

Other Figures

All Figure Page

Back to All Figure Page

Figure 4

PSD95-positive post-synaptic specializations in PyrN dendrites. (A) Maximum projection of PSD95-EGFP and DsRed expression in an isolated id2b:gal4 positive PyrN. Note discrete enrichments of PSD95-EGFP relative to cytosolic DsRed label. (A′) +50° Y-axis rotated view of image volume in (A). SM, SFGS, and SGC dendritic portions are indicated by boxed regions. Note presence of PSD95-EGFP puncta in SM, SFGS, and SGC layer dendrites. (B) −40° Y-axis rotated views of isolated subvolumes from boxed regions in (A′) showing PSD95-EGFP and DsRed expression in the SM, SFGS, and SGC dendrites. (C) Threshold images of PSD95-EGFP channel in rotated subvolumes in (B) as an example of threshold values used for automated particle analysis (puncta counting). (D–F) Quantification of PSD95-EGFP puncta number, neurite length, and puncta density for SM, SFGS, and SGC dendrite subregions in 7 fully reconstructed PyrNs obtained from 7 larvae. Scale bar: 20 μm in (A) and 15 μm in (B,C). *p < 0.05; **p < 0.001; ANOVA with Tukey's post-hoc test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Neuroanat.