FIGURE

Figure 2

ID

ZDB-FIG-210224-13

Publication

DeMarco et al., 2021 - Pyramidal Neurons of the Zebrafish Tectum Receive Highly Convergent Input From Torus Longitudinalis

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Figure 2

hspGGFF23C transgenic labels TL neurons that project to SM. (A,B) Low-resolution confocal images of 7 dpf triple transgenic Tg(hspGGFF23C,uas:egfp,isl2b:tagRFP) larva from side- and dorsal-view. (C) Higher magnification maximum projection images of TL and one tectal lobe of larva in (A,B). Merged fluorescence channel shown in (C), hspGGFF23C-driven EGFP channel shown in (C′). Note presence of EGFP-positive cells in TL and neurite plexus formed in tectal neuropil and lack of labeling in SPV layer of tectum. (D) Single confocal image through tectal neuropil. Note discrete EGFP labeling in the superficial SM layer of tectal neuropil. (E) Rotated side-view of image volume in (D), orientation orthogonal to the neuropil layers. (F) Fluorescence intensity profile measurement along line indicated by box in (E). Note peak in EGFP signal in the superficial SM layer. Scale bar: 300 μm in (A,B), 80 μm in (C), and 60 μm in (D,E).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Neuroanat.