Fig. 3. Trunk vascular alterations were enhanced by endogenous pdx1 expression silencing in aldh3a1−/− Tg(fli1:EGFP) zebrafish larvae. Endogenous pdx1 expression silencing by pdx1 morpholino injection led to enhanced formation of abnormal ISVs and hyperbranches in trunk vasculature of aldh3a1−/− Tg(fli1:EGFP) zebrafish larvae at 96 hpf. (A). Representative light microscopic images showed the gross morphology of zebrafish larvae and black boxes indicate region seen in the confocal images. White arrows indicate the abnormal ISVs and white deltas indicate the hyperbranches. White scale bar = 100 μm, black scale bar = 500 μm. (B–C). Quantification of abnormal ISVs and hyper branches formation in violin plots; n = 15–17 per group. 6 ng of morpholinos: Control-Mo and SB-pdx1-Mo were injected into the one-cell stage of zebrafish embryos respectively. For statistical analysis one-way ANOVA followed by Sidak's multiple comparison test was applied, *p < 0.05, **p < 0.01, ****p < 0.0001. pdx1, pancreatic and duodenal homeobox 1; ISVs, intersegmental vessels; Mo, morpholino; NS, not significant.
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