FIGURE

Fig 5

ID

ZDB-FIG-210216-21

Publication

Greenfeld et al., 2021 - The BMP signaling gradient is interpreted through concentration thresholds in dorsal-ventral axial patterning

Other Figures

All Figure Page

Back to All Figure Page

Fig 5

Thirty-minute duration of BMP2/7 sufficient for <italic>sizzled</italic>, <italic>foxi1</italic>, and <italic xmlns:xlink='http://www.w3.org/1999/xlink'>bambia</italic> target gene expression.

(A) Model of target gene expression regulated by distinct pSmad5 levels or distinct durations of BMP signaling. (B) Model of target gene activation after a 30-minute pulse of BMP ligand exposure. If target genes are activated by different pSmad5 levels, then all 3 target genes will be expressed following exposure to high levels of BMP signaling. If differences in signal duration activate BMP target gene expression, then a gene that requires a short signal duration will be expressed, but genes requiring longer signal durations will not be expressed. (C) Experimental schematic of a bmp7 mutant embryo injected with 5 pg of BMP2/7 protein that is fixed 30 minutes postinjection for pSmad5 immunostaining or FISH. (D) Representative immunostaining of pSmad5 intensities of an uninjected bmp7 mutant (n = 8) and a bmp7 mutant injected with 5 pg of BMP2/7 protein (n = 9). Animal pole is facing up. (E–G) Representative FISH in bmp7 mutants uninjected or injected with 5 pg of BMP2/7 protein for sizzled(E) (n = 11 uninjected, n = 11 injected), foxi1(F) (n = 10, n = 11), and bambia(G) (n = 10, n = 11). Animal pole is facing up. A.U. is arbitrary units. BMP, Bone Morphogenetic Protein; FISH, fluorescent in situ hybridization; hpf, hours post fertilization; pSmad5, phosphorylated Smad5; WT, wild-type.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Biol.