Fig. 6

Fig. 6. Effects of Mixture G and PFOS on apoptosis analyzed by TUNEL.A) The images show examples of terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) in the tail fins of zebrafish embryos exposed to vehicle (0.01% DMSO; left panel) or Mixture G at 100x hsc (right panel). The arrow heads indicate examples of labeled spots. The graphs show the relative amount of labeled spots B) at 54 hpf in embryos exposed to vehicle (?0?), Mixture G at 1, 20 or 100x hsc, or PFOS at 0.2, 2 or 10 µM and C) at 54, 78, and 102 hpf in embryos exposed to vehicle or Mixture G at 20x hsc. Embryo groups were exposed in triplicate glass petri dishes with exposure starting at 3 hpf. At the time for analysis all embryos of the same exposure were pooled to be fixed and processed for TUNEL. Data in B are from two combined experiments (n=21-35 embryos from 3 or 6 replicate dishes) and data in C are from embryos collected from the same triplicate dishes on three consecutive days (n=8-12 embryos). Data were normalized to the DMSO control means at 54 hpf. Each dot represents data from one embryo and the solid lines represent the group medians. The horizontal hatched lines indicate the DMSO medians at 54 hpf. Statistically significant differences between the DMSO and exposed groups were determined using Kruskal-Wallis test followed by Dunn?s test for selected pairs and are indicated by ? p?0.05 and ??? p?0.001.