FIGURE

FIGURE 4

ID
ZDB-FIG-210125-4
Publication
Khan et al., 2020 - Carbofuran accelerates the cellular senescence and declines the life span of spns1 mutant zebrafish
Other Figures
All Figure Page
Back to All Figure Page
FIGURE 4

Carbofuran accelerated autolysosomal puncta formation in a double‐transgenic zebrafish line of spns1 mutant background, expressing EGFP‐LC3 and mCherry‐Lamp1. A, Carbofuran exposure accelerated both autophagosomal EGFP‐LC3 and lysosomal mCherry‐Lamp1 expressions in spns1 mutant zebrafish. Lysosomal mCherry‐Lamp1 expression was merged to EGFP‐LC3 expression (bottom row; yellow colour). B, Quantification of autophagosomal EGFP‐LC3 expression. C, Quantification of lysosomal mCherry‐Lamp1 expression. D, Quantification of the co‐localization of expressions. Both EGFP‐LC3 and mCherry‐Lamp1 expressions (with their co‐localization) were significantly increased by the loss of the spns1 gene and were further significantly up‐regulated by carbofuran exposure. The scale bar is 10 mm; n = 6; *P ≤ .05; **P ≤ .01; ***P ≤ .005

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell. Mol. Med.