PUBLICATION
Carbofuran accelerates the cellular senescence and declines the life span of spns1 mutant zebrafish
- Authors
- Khan, A., Fahad, T.M., Akther, T., Zaman, T., Hasan, M.F., Islam Khan, M.R., Islam, M.S., Kishi, S.
- ID
- ZDB-PUB-201208-41
- Date
- 2020
- Source
- Journal of Cellular and Molecular Medicine 25(2): 1048-1059 (Journal)
- Registered Authors
- Keywords
- carbofuran, life span, senescence, spinster homolog 1, zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Autophagy/drug effects
- Autophagy/genetics
- Carbofuran/pharmacology*
- Cellular Senescence/drug effects*
- Cellular Senescence/genetics
- Gene Expression Regulation, Developmental/drug effects
- Longevity/drug effects
- Longevity/genetics
- Longevity/physiology*
- Lysosomes/drug effects
- Lysosomes/metabolism
- Membrane Proteins/genetics*
- Mutation/genetics*
- Phenotype
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Survival Analysis
- Zebrafish/genetics
- Zebrafish/growth & development
- Zebrafish/physiology*
- Zebrafish Proteins/genetics*
- beta-Galactosidase/metabolism
- PubMed
- 33277797 Full text @ J. Cell. Mol. Med.
Citation
Khan, A., Fahad, T.M., Akther, T., Zaman, T., Hasan, M.F., Islam Khan, M.R., Islam, M.S., Kishi, S. (2020) Carbofuran accelerates the cellular senescence and declines the life span of spns1 mutant zebrafish. Journal of Cellular and Molecular Medicine. 25(2):1048-1059.
Abstract
Carbofuran is a carbamate pesticide, widely used in agricultural practices to increase crop productivity. In mammals, carbofuran is known to cause several untoward effects, such as apoptosis in the hippocampal neuron, oxidative stress, loss of memory and chromosomal anomalies. Most of these effects are implicated with cellular senescence. Therefore, the present study aimed to determine the effect of carbofuran on cellular senescence and biological ageing. Spinster homolog 1 (Spns1) is a transmembrane transporter, regulates autolysosomal biogenesis and plays a role in cellular senescence and survival. Using senescence-associated β-galactosidase staining, we found that carbofuran accelerates the cellular senescence in spns1 mutant zebrafish. The yolk opaqueness, a premature ageing phenotype in zebrafish embryos, was accelerated by carbofuran treatment. In the survival study, carbofuran shortened the life span of spns1 mutant zebrafish. Autophagy is the cellular lysosomal degradation, usually up-regulated in the senescent cells. To know the impact of carbofuran exposure on autophagy progress, we established a double-transgenic zebrafish line, harbouring EGFP-tagged LC3-II and mCherry-tagged Lamp1 on spns1 mutant background, whereas we found, carbofuran exposure synergistically accelerates autolysosome formation with insufficient lysosome-mediated degradation. Our data collectively suggest that carbofuran exposure synergistically accelerates the cellular senescence and affects biological ageing in spns1 defective animals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping