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Fig. 5

ID

ZDB-FIG-201209-25

Publication

H�gset et al., 2020 - In vivo biomolecular imaging of zebrafish embryos using confocal Raman spectroscopy

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Fig. 5

Biomolecular profiling and discrimination of mycobacterial infections.

a Volumetric confocal Raman spectroscopic imaging (cRSI) of zebrafish embryos infected with either wild type or ∆RD1 M. marinum or injected with PBS as a negative control. cRSI was performed at 4 days post injection, and 3D reconstructions show embryo tissue in gray with mycobacterial clusters displayed in red (wild type) or blue (∆RD1). Scale bars: 50 µm. b Mean spectra of the mycobacterial clusters extracted from the volumetric cRSI scans for wild type M. marinum (red trace) and the ∆RD1 mutant bacteria (blue trace). c Principal component analysis showed clear separation of the wild type M. marinum (red markers) and the ∆RD1 mutant bacteria (blue markers) using the three principal components (PC1, PC2, PC3).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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