ZFIN Image: Høgset et al., 2020, Fig. 5

Image

Figure Caption

Fig. 5 Biomolecular profiling and discrimination of mycobacterial infections.

a Volumetric confocal Raman spectroscopic imaging (cRSI) of zebrafish embryos infected with either wild type or ∆RD1 M. marinum or injected with PBS as a negative control. cRSI was performed at 4 days post injection, and 3D reconstructions show embryo tissue in gray with mycobacterial clusters displayed in red (wild type) or blue (∆RD1). Scale bars: 50 µm. b Mean spectra of the mycobacterial clusters extracted from the volumetric cRSI scans for wild type M. marinum (red trace) and the ∆RD1 mutant bacteria (blue trace). c Principal component analysis showed clear separation of the wild type M. marinum (red markers) and the ∆RD1 mutant bacteria (blue markers) using the three principal components (PC1, PC2, PC3).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.