A20 is necessary to maintain inflammatory homeostasis in zebrafish. (A) Representative fluorescent image of a NF-κB:EGFP zebrafish at 6 dpf showing spontaneous NF-κB activity (fluorescence) in the (a) mouth, (b) lateral neuronmasts, (c) pharyngeal tooth, and (d) gills. Scale bar represents 2 mm. (B) Representative fluorescent images of NF-κB:EGFP zebrafish of different A20 genotypes at 3 and 6 dpf ± LPS. The scale bars represent 1 mm. (C, D) Cumulative data of relative fluorescence units of NF-κB:EGFP zebrafish by A20 genotype at; 3 dpf without LPS stimulation and 6 dpf ± LPS. Each dot represents an individual fish. Data are representative of N = 10 crosses. N per group and mean values reported below each graph. Data represented as mean ± SD, P-values were determined using 1-way ANOVA. Each group passed the normality test. **P < 0.01, ***P < 0.001, ****P < 0.0001. Software used for this Figure: GraphPad Prism v7.0 (https://www.graphpad.com/scientific-software/prism/), Adobe Illustrator CC 2018 (https://www.adobe.com/au/products/illustrator.html), Leica LAS X (https://www.leica-microsystems.com/products/microscope-software/p/leica-application-suite/).
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