Fig. 5

Pcdh18a affects E-cadherin dependent cell migration. a Wound-healing assay in HeLa cells. Cells were transfected with the indicated constructs and their migratory behaviour was monitored for 10 h after removing the insert. The dotted line shows limits of the confluent cell layer. b Quantification of the migration speed of HeLa cells. c Wound-healing assay in L cells. Cells were transfected with indicated constructs. After 24 h, L cells were treated with DMSO (1%) or Dyngo4a endocytosis inhibitor (1 µM in DMSO 1%). The migratory behaviour of cells was monitored in a time lapse for 34 h. d Quantification of the migration speed of L cells after blocking endocytosis. All wound-healing assays were conducted in independent triplicate, distances of the gap were measured at ten fixed positions. Mean values, SEM and significance are indicated (*p value < 0.05, ***p value < 0.005; unpaired Student’s t test). Scale bar: 200 µm