FIGURE

Figure 8.

ID

ZDB-FIG-201012-81

Publication

Inaba et al., 2020 - Keratins and Plakin family cytolinker proteins control the length of epithelial microridge protrusions

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Figure 8.

The periplakin tail domain is required to stabilize microridges.

(A) Superimposition of first and last frames from a time-lapse movie of WT cells expressing Lifeact-GFP at the indicated developmental stages. Green: Starting time point, Magenta: 4.5 min time point. Overlap is white. (B) Line plots of overlap coefficients at the indicated stages, comparing each time point to the first. n = 21–33 cells from 4 to 5 fish per category. Bars = 95% confidence interval. (C) Superimposition of first and last frames from a time-lapse movie of evpl^−/−;ppl^−/− double mutant animals expressing Lifeact-GFP, rescued with the indicated Evpl and Ppl fusions. (D) Line plots of overlap coefficients in cells expressing the indicated Evpl and Ppl fusions, comparing each time point to the first. n = 11 cells from 3 to 4 fish per category. Bars = 95% confidence interval. (E) Two-step model of microridge morphogenesis. First, plakins interact with F-actin via their head domains to fuse pegs into short microridges. Second, plakin-keratin interactions through plakin tail domains stabilize microridges, allowing them to further elongate. Scale bars: 10 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	evpla^la230/la230; ppl^la229/la229; la227Tg/la227Tg
Observed In:	peridermal cell keratin filament peridermal cell keratin filament binding
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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