Figure 5—figure supplement 1.

Evpl depends on Ppl for apical localization, but not vice versa.

(A) Ppl-GFP[BAC]- and Lifeact-mRuby-expressing WT and evpl−/− periderm cells. Ppl localized to microridges in WT and pegs/short microridges in evpl−/− periderm cells at 48hpf. (B) Evpl-GFP[BAC]- and Lifeact-mRuby-expressing WT and ppl−/− periderm cells at 48hpf. Evpl localized to microridges in WT and to the cytoplasm in ppl−/− cells. Black-and-white images were inverted so that high-intensity fluorescence appears black and low-intensity fluorescence is white. Scale bars: 10 µm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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