FIG 4

Mimicry embedding can be used for weak-labeling particle classification. (A) Merged four channel fluorescent image of a HeLa cell infected with VACV previously unseen by CapsNet (see Fig. S4A for channel details). (B) ZedMate detection and trained-CapsNet-predicted extracellular and intracellular particles. (Inset) Quantification of intracellular particles. (C) Merged four-channel image of HeLa cell infected with VACV and treated with the entry inhibitor IPA-3, previously unseen by CapsNet. (D) ZedMate detection and trained CapsNet inference of intracellular and extracellular particles. (Inset) Quantification of intracellular particles. (E) Representative reconstruction profiles of extra- and intracellular virions. (F) Representative mimicry-embedded extra- and intracellular VACV particles for comparison to the images in panel E. n = 40 (3 biological replicates) untreated and treated cells each. Detailed model performance (statistical validation) metrics are provided in Fig. S4. Bars, 10 μm.