FIGURE

Figure 2.

ID

ZDB-FIG-200904-19

Publication

Hu et al., 2020 - Loss of the RNA-binding protein Rbm15 disrupts liver maturation in zebrafish

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Figure 2.

The mutation gene in cq96 is rbm15.A and B, schematic drawing of the rbm15 locus, cq96, CRISPR/Cas9 targeting site (green), and the PAM sequence (red). C, genomic sequencing result of rbm15 mutation derived from the CRISPR/Cas9 knockout experiment. D, WISH and fluorescence in situ hybridization (FISH) results showing the expression pattern of rbm15 from 24 hpf to 5 dpf. E, heat shock transgenic line Tg(hsp70l:rbm15-Flag^cq97) rescues liver defects of cq96; confocal images show the expression of Dendra2. F, the quantification of fluorescent intensity of Dendra2 in the liver. G, evaluating the rescue effects by detecting the expression of liver functional genes uox and gc via WISH. Numbers indicate the proportion of larvae exhibiting the expression shown. Asterisks indicate statistical significance: ****, p < 0.0001. Scale bars, 100 μm; error bars, S.D.

Expression Data

Genes:	cp Dendra2 rbm15 uox
Fish:	WT rbm15^cq96/cq96 rbm15^cq96/cq96; cq1Tg rbm15^cq96/cq96; cq97Tg rbm15^cq96/cq96; cq1Tg; cq97Tg
Condition:	heat shock
Anatomical Terms:	liver liver primordium
Stage Range:	Prim-5 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rbm15^cq96/cq96 rbm15^cq96/cq96; cq1Tg
Condition:	heat shock
Observed In:	liver
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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