ZFIN ID: ZDB-FIG-200829-92
Min et al., 2020 - Systems Analysis of Biliary Atresia Through Integration of High-Throughput Biological Data. Frontiers in Physiology   11:966 Full text @ Front. Physiol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 5

FIGURE 7

Activation of Hif1a signaling increases BEC number and impairs the proper formation of bile canaliculi. (A) Confocal projection images showing intrahepatic biliary network by Tp1:GFP expression in the liver (dotted lines) at 5 dpf. Boxed regions are enlarged. (B) Confocal projection images showing Tp1:H2B-mCherry expression (red; BEC nuclei) in the liver (dotted lines) at 5 dpf. Quantification of BEC number is shown. (C) Confocal projection images showing Abcb11 and Tp1:GFP expression in the liver (dotted lines) at 5 dpf. Arrowheads point to bile canaliculi. n indicates the number of larvae used for quantification. Scale bars: 50 μm; error bars: ± SD.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP um14Tg standard conditions Day 5 intrahepatic bile duct epithelial cell cytoplasm IFL
mCherry s939Tg standard conditions Day 5 intrahepatic bile duct epithelial cell nucleus IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s939Tg chemical treatment by environment: dimethyloxalylglycine Day 5 intrahepatic bile duct epithelial cell increased amount, abnormal
um14Tg chemical treatment by environment: dimethyloxalylglycine Day 5 bile canaliculus decreased length, abnormal
Day 5 bile canaliculus increased amount, abnormal
Day 5 intrahepatic bile duct epithelial cell increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in Physiology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front. Physiol.