FIGURE

FIGURE 6

ID

ZDB-FIG-200829-41

Publication

Muchaamba et al., 2020 - Evolution of Listeria monocytogenes During a Persistent Human Prosthetic Hip Joint Infection

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FIGURE 6

Strains vary in virulence and persistence within zebrafish: (A) In zebrafish embryos infected using the blood stream injection route, both N843_15 and N843_10 were unable to induce any clinical signs or mortality 72 h post infection. The reference strains used N2306 (CC4) and LL195 (CC1) had induced 100%, whilst EGDe (CC9) and N12-1273 (CC412) had induced 100% and 50% mortality of the zebrafish embryos within 24 and 72 h of infection, respectively. (B) GFP zebrafish embryo line coinfected with N843_15 mO2FP and N843_10 GFP. The parent strain N843_10 decreased in CFU numbers over time consistent with it being cleared while N843_15 levels remained unchanged over 48 h of infection. Results show the mean and standard deviation from 3 independent experiments. ^∗Indicates significant differences in the level of the two strains at 24 and 48 hpi (P < 0.05 based on repeated measures two-way ANOVA).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WIK
Condition:	bacterial treatment by injection: Listeria monocytogenes
Observed In:	whole organism
Stage:	Day 5

Phenotype Detail

Acknowledgments

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