FIGURE

Fig. 4

ID

ZDB-FIG-200829-4

Publication

Satapathy et al., 2020 - Tumour suppressor 15-hydroxyprostaglandin dehydrogenase induces differentiation in colon cancer via GLI1 inhibition

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Fig. 4

15-PGDH promotes differentiation in colon cancer via GLI1.

qRT-PCR validation of gene expression in shCTRL (control shRNA)- or shGLI1 (GLI1-specific shRNA)-transfected HT-29 cells with or without LTC₄ stimulation for 48 h. Marker of tumour suppression in a15-PGDH. Markers of differentiation in bSI, in cMUCIN-2, and in dCDHR2. Marker of differentiation regulation as in eCDX2. Markers of Wnt activation in fAXIN2 and gcMYC. Marker of proliferation in h (CCND1). HPRT1 was used as the housekeeping gene for normalization of the gene expression data. i Western blot analysis of whole-cell lysates for 15-PGDH, GLI1, SI, CDX2, and CDHR2 expression in shCTRL (control shRNA)- or shGLI1 (GLI1-specific shRNA)-transfected HT-29 cells with or without LTC₄ stimulation for 48 h. α-Tubulin served as the loading control. j Immunofluorescence analysis of GLI1 and Mucin-2 in cells with or without LTC₄ stimulation for 48 h and transfected with shCTRL or shGLI1. Graphs represent the mean ± SEM of data from three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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